ABSTRACT
Objective To investigate the bacterial community diversity in Dermatophagoides farinae. Methods Laboratory-cultured D. farinae was collected, and the composition of microbial communities was determined by sequence analyses of the V4 region in the bacterial 16S ribosomal RNA (16S rRNA) gene on an Illumina PE250 high-throughput sequencing platform. Following quality control and filtering of the raw sequence files, valid reads were obtained and subjected to operational taxonomic units (OTU) clustering and analysis of the composition of microbial communities and alpha diversity index using the Usearch software, Silva database, and Mothur software. Results A total of 187 616 valid reads were obtained, and 469 OTUs were clustered based on a sequence similarity of more than 97%. OTU annotation showed that the bacteria in D. farinae belonged to 26 phyla, 43 classes, 100 orders, 167 families and 284 genera. The bacteria in D. farinae were mainly annotated to five phyla of Proteobacteria, Firmicutes, Bacteroidota, Actinobacteriota, and Acidobacteriota, with Proteobacteria as the dominant phylum, and mainly annotated to five dominant genera of Ralstonia, norank-f-Mitochondria, Staphylococcus and Sphingomonas, with Wolbachia identified in the non-dominant genus. Conclusions A high diversity is identified in the composition of the bacterial community in D. farinae, and there are differences in bacterial community diversity and abundance among D. farinae.
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Objective To establish a nursing risk assessment index system (NRAIS) for stroke-associated pneumonia (SAP), and provide reference for the prevention and control of SAP.Methods On the basis of literature analysis and semi-structured interviews, the basic framework of NRAIS for SAP was preliminarily drawn up. Indexes were screened by using Delphi method and analytic hierarchy process, the weight of index was calculated.Results The NRAIS for SAP consisting of 6 first-level indexes and 33 second-level indexes was constructed, positive coefficient of expert of two rounds consultation were 88.89％ and 100％ respectively, authoritative coefficients was 0.8％.Conclusion The constructed assessment index system can effectively help managers identify high-risk factors and high-risk links of SAP, and provide evaluation basis for effective prevention of clinical nursing risks.
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the expression of human anti-apoptotic gene survivin (SVV) in normal human gastric tissues and gastric cancer.</p><p><b>METHODS</b>SVV cDNA clone was obtained from human gastric cancer tissues by virtue of RT-PCR, using Dig-marked cRNA probe in situ hybridization to analyze its expression in normal human gastric tissues and gastric cancer.</p><p><b>RESULTS</b>Two SVV cDNA clones, SVV-S4A and SVV-S1B were obtained. The sequence of the former is identical to that of the well-known SVV cDNA; however, in the sequence of the latter, the third exon was missed, i.e., there are only two exons in SVV-S1B. In situ hybridization showed that SVV-S4A is mainly expressed in gastric cancer tissues whereas SVV-S1B is mostly expressed in normal gastric tissues.</p><p><b>CONCLUSION</b>There is difference between SVV-S4A and SVV-S1B in respect to their characteristics of expression in gastric cancer and normal gastric tissues.</p>